Marcos de Moraes

WEBSITE(S)| de Moraes Lab

SURF Mentoring

Potential projects/topics: One of the main research topics in my lab is the study of bacteria to bacteria interactions mediated by delivery of toxins. The undergrads will be work in a project identifying are are the requirements for these bacterial interactions. Specifically, the students would test different bacterial mutants, previously constructed, in competition experiments between two different bacterial genotypes. These experiments allow the identification of genes required for bacteria to bacteria interactions in microbial community models. Other experiments would include the characterization of the mutants in growth curves and basic molecular biology methods such as PCR. We have been successful integrating undergrads with this experimental approach. It allows the students to have a testable hypothesis and it gives them a sense of accomplishment by resulting in a clear outcome.

Potential skills gained: General microbiology, culture methods, bacterial isolation, moelcular biology, PCR, gel electrophoresis, data analysis, statistics, and graphic visualization of data

Required qualifications or skills: Entry level biology courses

Direct mentor: Post-doctorate, Other Research Associate

Research Areas

Bacteria are found in most environments where they form communities with a complex interaction network of cooperation and antagonism. It is also in the context of communities that bacteria undergo a process of adaptation to cope with changing environmental conditions. To defend their niche and guarantee access to limited resources, bacteria can employ toxin delivery pathways that target competing cells. Through the study of bacterial toxins and their long-term physiological effects, the de Moraes Lab aims to elucidate how interactions shape microbial communities assembly, composition, and evolution. Moreover, the de Moraes lab is interested in harnessing the potential of bacterial toxins for biotechnological applications.